Featured Technology Archive

Summer 2013

Chaperone-Assisted Biology: Versatile Approaches by Two PSI:Biology Partnerships

Two PSI:Biology Partnerships are developing three different types of antibody-related chaperones for structure and function studies.

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*Crystal structure of Saccharomyces cerevisiae NUP192, residues 2 to 960 [ScNup192(2-960)], from Sampathkumar P, Kim SJ, Upla P, Rice, WJ, Phillips J, Timney BL, Pieper U, Bonanno JB, Fernandez-Martinez J, Hakhverdyan Z, Ketaren NE, Matsui T, Weiss TM, Stokes DL, Sauder JM, Burley SK, Sali A, Rout MP, Almo SC. "Structure, dynamics, evolution, and function of a major scaffold component in the nuclear pore complex." Structure 21:560-571 (2013). Pubmed ID:23499021 | PMC Link

Fall 2012

In crystallo and in silico Approaches to Functional Proteomics

A new high-throughput experimental method joins the many computational tools developed by PSI in furthering the area of functional proteomics.

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*Structure of ADP/ATP-dependent NAD(P)H-hydrate dehydratase from Bacillus subtilis from Shumilin IA, Cymborowski M, Chertihin O, Jha KN, Herr JC, Lesley SA, Joachimiak A, Minor W. "Identification of unknown protein function using metabolite cocktail screening." Structure 20:1715-1725 (2012). Pubmed ID:22940582 | Search SBKB Publications Portal

Summer 2012

Leveraging the Power of Molecular Fragment Replacement to Solve Protein Structures by NMR

Two PSI Centers, NESG and MPSbyNMR have used a technique pioneered by x-ray crystallographers to advance the field of automatic NMR structure determination and solve the structure of a eukaryotic transmembrane protein, respectively.

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*Structure of Alg13 from Wang X, Weldeghiorghis T, Zhang G, Imperiali B, Prestegard JH. "Solution structure of Alg13: the sugar donor subunit of a yeast N-acetylglucosamine transferase." Structure 16:965-75, 2008. Pubmed ID:18547528 | Search SBKB Publications Portal | PMC Link

Winter 2011

Membrane Proteins of Known 3D Structure Database: The Go-To Resource for Membrane Protein Structures and Hydropathy Information

Started in 1998 by Professor Stephen White at UC-Irvine, the Membrane Proteins of Known 3D Structure Database has evolved to house over 300 structures, a bulletin board for posting PDB-released structures prior to publication, and two other membrane protein-related tools, MPtopo and MPEx.

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Spring 2011

Automating the Structure Determination Pipeline: Advances by Two Membrane Protein Centers

3PJZIn the burgeoning field of membrane protein structure determination, two of the PSI:Biology Centers for Membrane Protein Structure Determination have developed automation and robotic technology to establish efficient and productive structure determination pipelines.

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*Structure of the TrkH potassium transporter from Cao Y, Jin X, Huang H, Derebe MG, Levin EJ, Kabaleeswaran V, Pan Y, Punta M, Love J, Weng J, Quick M, Ye S, Kloss B, Bruni R, Martinez-Hackert E, Hendrickson WA, Rost B, Javitch JA, Rajashankar KR, Jiang Y, Zhou M. "Crystal structure of a potassium ion transporter, TrkH." Nature 471:336-40, 2011. Pubmed ID:21317882 | Search SBKB Publications Portal

Fall 2010

Redesigned BioSync Website is a Valuable Resource for Structural Biologists


Originally started as a website to provide basic information about the small number of synchrotron beamlines used for macromolecular crystallographers, BioSync has metamorphosed into a structural biologists' guide to high-energy data collection facilities. This newly redesigned web resource includes detailed information about the more than 130 beamlines worldwide that are used to collect experimental data on biological macromolecules. The beamline scientists provide information on the actual conditions of the beamline for data collection and the services offered to users (e.g., remote data collection and robotics for crystal screening and mounting).

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Spring 2010

Reconstitution of a Three-Component Membrane Protein Complex Using Wheat Germ Cell-Free Technology

MP fold

Wheat germ cell-free translation has been shown to be an effective method to produce integral membrane proteins in the presence of unilamelar liposomes. A three-component membrane protein complex was reconstituted using wheat germ cell-free technology. The data strongly support the utility of wheat germ cell-free technology to produce active and correctly folded integral membrane proteins.

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