Center for Membrane Proteins in Infectious Diseases
Plant-optimized HIV-1 genes were constructed and expressed in Nicotiana benthamiana. Our results demonstrate plant cells could support the formation of HIV-1 virus-like particles, but also the accumulation of these particles that incorporated dgp41.
Center for Membrane Proteins in Infectious Diseases
Screening for crystallisability based on the identity of the lipid creating the hosting mesophase would be worthwhile. Host lipid screening is likely to prove a generally useful strategy for mesophase-based crystallisation of membrane proteins.
Center for Membrane Proteins in Infectious Diseases
The use of lipidic mesophases, both cubic and sponge phases, for growing integral membrane crystal proteins has grown in popularity. Protocols for harvesting and cryo-cooling crystals grown in these two different phases are described in detail in this JoVE article (Li, et al., 2012).
Center for Membrane Proteins in Infectious Diseases
A systematic study of the crystallization of an α-helical, integral membrane enzyme, diacylglycerol kinase, DgkA, using the lipidic cubic mesophase or in meso method has resulted in recommended screening strategies for crystallizing membrane proteins.
Center for Membrane Proteins in Infectious Diseases
We prepared nano-sized in vivo–grown crystals of Trypanosoma brucei enzymes and applied the emerging method of free-electron laser-based serial femtosecond crystallography to record interpretable diffraction data. This combined approach will open new opportunities in structural systems biology.
